An estimated 150,000 first-time buyers have benefited from the increase in the threshold over the past two years with approximately 380,000 first-time buyers paying no stamp duty.
Returning the starting threshold to £125,000 will result in nearly 45% of first-time buyers paying stamp duty.
The South East and Greater London have benefited most from the change with around seven in ten first-time buyers having to pay no stamp duty directly due to the raising of the threshold from £125,000 to £250,000.
The North and Northern Ireland have benefited least with only one in six first-time buyers exempt from the tax as a result of the increase.
Only 4% of first-time buyers in Greater London will be exempt from stamp duty when the starting threshold returns to £125,000. Four in five first-time buyers in the South East will pay the tax.
The proportions of first-time buyers paying stamp duty will be much lower in other parts of the country with less than one in five paying the tax in the North and Northern Ireland.
Martin Ellis, Halifax housing economist, said: "The stamp duty holiday has directly helped four in ten first-time buyers over the past two years. Regionally, the impact has varied considerably with around seven in ten first-time buyers in London and the South East exempt from the tax as a direct result of the temporary change. Returning the threshold to £125,000 will hit those buying their first home in these parts of the country most.
"The doubling in the starting threshold has been a significant benefit to those who have benefited during a time of economic and financial difficulty for many. A first-time buyer making a purchase of £200,000 will see their buying costs increase by £2000."
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